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crispr constructs plos genetics  (Addgene inc)


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    Structured Review

    Addgene inc crispr constructs plos genetics
    Crispr Constructs Plos Genetics, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+constructs+plos+genetics/pDD2028+(Plasmid+%23101371)/pm41843552-187-0-25
    Average 93 stars, based on 37 article reviews
    crispr constructs plos genetics - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    CRISPR:

    Article Title: Functional interrogation of candidate cis-regulatory elements at the LDLR locus.
    Article Snippet: Oligonucleotide sequences (Integrated DNA Technologies, Coralville IA) are provided in S10 Table. .. CRISPR constructs PLOS Genetics | https://doi.org/10.1371/journal.pgen.1012082 March 17, 2026 13 / 21 were generated by ligation of phosphorylated and annealed oligonucleotides into BsmBI-digested pLentiCRISPRv2 [61] (Addgene, Watertown MA, #52961) or pX459 [62] (Addgene #62988) as previously described [63]. .. Reporter constructs were generated by PCR amplification of the indicated noncoding sequence from genomic DNA or a plasmid template with primers designed to provide flanking homology arms for HiFi assembly into AgeI/SbfI-digested (New England Biolabs, Ipswich MA) pLS-SceI30 (Addgene #137725) as previously described [30].

    Construct:

    Article Title: Functional interrogation of candidate cis-regulatory elements at the LDLR locus.
    Article Snippet: Oligonucleotide sequences (Integrated DNA Technologies, Coralville IA) are provided in S10 Table. .. CRISPR constructs PLOS Genetics | https://doi.org/10.1371/journal.pgen.1012082 March 17, 2026 13 / 21 were generated by ligation of phosphorylated and annealed oligonucleotides into BsmBI-digested pLentiCRISPRv2 [61] (Addgene, Watertown MA, #52961) or pX459 [62] (Addgene #62988) as previously described [63]. .. Reporter constructs were generated by PCR amplification of the indicated noncoding sequence from genomic DNA or a plasmid template with primers designed to provide flanking homology arms for HiFi assembly into AgeI/SbfI-digested (New England Biolabs, Ipswich MA) pLS-SceI30 (Addgene #137725) as previously described [30].

    Generated:

    Article Title: Functional interrogation of candidate cis-regulatory elements at the LDLR locus.
    Article Snippet: Oligonucleotide sequences (Integrated DNA Technologies, Coralville IA) are provided in S10 Table. .. CRISPR constructs PLOS Genetics | https://doi.org/10.1371/journal.pgen.1012082 March 17, 2026 13 / 21 were generated by ligation of phosphorylated and annealed oligonucleotides into BsmBI-digested pLentiCRISPRv2 [61] (Addgene, Watertown MA, #52961) or pX459 [62] (Addgene #62988) as previously described [63]. .. Reporter constructs were generated by PCR amplification of the indicated noncoding sequence from genomic DNA or a plasmid template with primers designed to provide flanking homology arms for HiFi assembly into AgeI/SbfI-digested (New England Biolabs, Ipswich MA) pLS-SceI30 (Addgene #137725) as previously described [30].

    Ligation:

    Article Title: Functional interrogation of candidate cis-regulatory elements at the LDLR locus.
    Article Snippet: Oligonucleotide sequences (Integrated DNA Technologies, Coralville IA) are provided in S10 Table. .. CRISPR constructs PLOS Genetics | https://doi.org/10.1371/journal.pgen.1012082 March 17, 2026 13 / 21 were generated by ligation of phosphorylated and annealed oligonucleotides into BsmBI-digested pLentiCRISPRv2 [61] (Addgene, Watertown MA, #52961) or pX459 [62] (Addgene #62988) as previously described [63]. .. Reporter constructs were generated by PCR amplification of the indicated noncoding sequence from genomic DNA or a plasmid template with primers designed to provide flanking homology arms for HiFi assembly into AgeI/SbfI-digested (New England Biolabs, Ipswich MA) pLS-SceI30 (Addgene #137725) as previously described [30].



    Similar Products

    crispr constructs plos genetics  (Addgene inc)
    93
    Addgene inc crispr constructs plos genetics
    Crispr Constructs Plos Genetics, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+constructs+plos+genetics/pDD2028+(Plasmid+%23101371)/pm41843552-187-0-25
    Average 93 stars, based on 1 article reviews
    crispr constructs plos genetics - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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